Fig. 3.

Proton uptake from solution during the flow-flash reaction of the fully reduced solubilized cbb₃ with either O₂ or NO. Experimental details as in Fig. 1 except that no buffer was present, the mixing was 1:5, and the solution contained 0.03% DDM. The traces shown are differences where the trace in the presence of 50 mM Hepes has been subtracted from the trace in the absence of buffer.