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. Author manuscript; available in PMC: 2009 Jan 21.
Published in final edited form as: Cell. 2008 Dec 26;135(7):1201–1212. doi: 10.1016/j.cell.2008.11.042

Figure 6. AID and hMbd4 Occupy the neurod2 Promoter and Affect the Methylation Status at 80% Epiboly.

Figure 6

(A) Schematic of the neurod2 promoter and start site region. R1 and R2 show regions of bisulfite sequencing (Results shown for only R1; R2 remains unmethylated and unaffected). P1 and P2 depict the amplicons used for ChIP determinations.

(B) Bisulphite sequencing of the R1 region of the neurod2 promoter in the WT (uninjected) animals or animals injected with morpholinos (all 2 pg) as shown. Scr Mo; a control morpholino where the base composition is maintained, but the order scrambled.

(C) Enrichment of AID and hMbd4 at neurod2 (P1 versus P2). ChIP experiments with extracts from embryos at 80% epiboly, which were initially injected at the single-cell stage with V5-tagged AID and HA-tagged hMbd4. Graph shows one representative biological experiment (two biological repeats), with the average of three technical replicates shown. Error bars are ± one standard deviation.