Figure 5.
(A) Results of UV-B irradiation and Bay 11-7085 on apoptosis as determined by Annexin V staining. Inhibition of NF-κB activity with Bay11-7085 sensitizes BAR-T cells to apoptosis induced by low dose UV-B in a dose dependent manner. The bar graphs depict the mean + SEM of at least 3 individual experiments. (*, p<0.001 compared to non-irradiated corresponding controls; +, p<0.001 compared to 1 μM; #, p<0.001 and &, p<0.01 compared to 2.5 μM, @, p<0.001 compared to 5 μM). (B) Western blots for NF-κB/p65 expression following siRNA infection and results of UV-B irradiation and NF-κB/p65 siRNA on apoptosis as determined by Annexin V staining. Note that inhibition of NF-κB/p65 sensitizes BAR-T cells to apoptosis induced by low dose UV-B irradiation. SiRNA against lamin A/C served as a control. The bar graphs depict the mean + SEM of at least 3 individual experiments. (*, p<0.001 compared to non-irradiated corresponding controls)