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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: Cancer Res. 2009 Jan 15;69(2):709–717. doi: 10.1158/0008-5472.CAN-08-3415

Figure 2.

Figure 2

(A) Graph showing enrichment in the three HA-SOX4 lanes over the average of the two YFP replicates for the SOX4 target gene FMO4 Y-axis is the signal intensity across the genomic coordinates on the X-axis. (B) qPCR ChIP analysis of 10 randomly selected genes verified in both the RWPE-1 and LNCaP cell lines. Graph shows fold enrichment of the HA-SOX4 IP over the YFP negative control IP. Numbers above the bars represent the mean log2 of fold enrichment of ChIP-chip signal for the probes contained in the peak relative to YFP. Error bars indicate 1 SD (n = 3 technical replicates). (C) and (D) Genes that were verified by conventional ChIP assay. HA-SOX4 and YFP cells were subjected to conventional ChIP followed by PCR in both the LNCaP (C) and RWPE-1 (D) prostate cell lines. Six genes verified in the LNCaP cell lines and five in the RWPE-1 cell lines.