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. 2009 Jan 21;106(5):1439–1444. doi: 10.1073/pnas.0811268106

Fig. 3.

Fig. 3.

Localization of the psbA mRNA for the de novo assembly and repair of PSII. (A–E) Fluorescence signals from the psbA mRNA and the chloroplast ribosomal protein S-21 in cells from the following conditions: a 2-h DA (A); DA cells exposed to HL for 1 min (HL1′) to induce psbA translation for de novo PSII assembly (B); HL1′ cells generated in the presence of lincomycin (C); HL1′ cells exposed to ML for 5 min, a condition of PSII repair (HL1′ML5′) (D); and HL1′ML5′ cells generated in the presence of lincomycin (E). The micrographs show 0.2-μm optical sections. (Scale bar: 1 μm.) (F) The percentages of pixels with strong colocalized signals in T zones (white bars) and chloroplast lobes (shaded bars) across all cells from the 5 conditions. The error bars indicate 2 standard errors. For each experiment, n ≥ 20 cells.