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. 2009 Jan 21;106(5):1439–1444. doi: 10.1073/pnas.0811268106

Fig. 4.

Fig. 4.

FISH analyses of 2 nucleocytosolic mRNAs. (A) An ML cell showing the distribution of the LhcII mRNAs relative to the chloroplast, stained by immunolabeling L2. The closed-head arrows indicate colocalization near the chloroplast basal region. (B) An ML cell showing the fluorescence signals from LhcII mRNAs and the cytoplasmic ribosomal protein cyL4. (C) An ML cell exposed to puromycin for 1 min that was FISH-probed for the LhcII mRNAs and immunostained for cyL4. (D) An ML cell showing the distribution of the RbcS2 mRNAs relative to the chloroplast, which was FISH-probed for the psbC mRNA. (E) An ML cell that was FISH-probed for the RbcS2 mRNA and immunostained for the cytoplasmic ribosomal protein cyL4. The open-head arrow shows the autofluorescence resulting from excitation at 633 nm, seen in the nonprobed/immunostained cell in (F). (G) An ML cell that was FISH-probed for LhcII and RbcS2 mRNAs. DIC, differential interference contrast. The micrographs show 0.2-μm optical sections. (Scale bar: 1 μm.)