Table 2.
Details of primers and amplicons for each of the 11 evaluated genes
| Gene Symbol |
Oligo Sequence Forward/Reverse |
Arabidopsis targeted Exons |
Optimized Primer Concentration [μM] |
Amplicon Length (pb)/Tm |
Efficiency | ||
| cDNA* | gDNA** | Mean*** | SD | ||||
| GAPDH | GGCTGCAATCAAGGAGGAA/AAATCAATCACACGGGAACTG | 9th/10th 11th |
0.2 | 207/78.1 | N/A | 0.913 | 0.027 |
| EFα1 | TACTGGTGGTTTTGAAGCTG/AACTTCCTTCACGATTTCATCATA | 2nd 3rd |
0.2 | 166/79.2 | 246/79.2 | 0.953 | 0.106 |
| TBP | GCTAAGAACGCTGGACCTAATG/TGGGTGTGCCTTTCTGAATG | 4th 6th |
0.6 | 184/76.1 | N/A | 0.959 | 0.044 |
| RPL8 | CCGAAGGAGCTGTTGTTTGTA/ACCTGACCAATCATAGCACGA | 1st 2nd |
0.2 | 184/79.3 | N/A | 0.902 | 0.026 |
| APT | CCATGAGGAAACCCAAGAAGT/CCTCCAGTCGCAATTAGATCAT | 4th 5th |
0.2 | 143/78.5 | 1150/75.2 | 0.887 | 0.024 |
| DNAJ | GAGCACACATTGAGCCTTGAC/CTTTGGTACATCGGCATTCC | 5th 6th |
0.2 | 158/79.6 | 570/76.80 | 0.880 | 0.028 |
| TUA | AGCTCATTAGCGGCAAAGAA/AGTACCCCCACCAACAGCA | 2nd 3rd |
0.2 | 163/77.0 | 254/78.60 | 0.973 | 0.106 |
| TIP41 | ATGGAGTTTTTGAGTCTTCTGC/GCTGCGTTTCTGGCTTAGG | 6th/7th 8th |
0.4 | 235/78.3 | 1157/80.2 | 0.941 | 0.053 |
| SAND | TTGCTTGGAGGAACAGACG/GCAAACAGAACCCCTGAATC | 6th 7th |
0.4 | 164/78.2 | N/A | 0.944 | 0.053 |
| CAC | CCTCCGTTGTGATGTAACTGG/ATTGGTGGAAAGTAACATCATCG | 7th 8th |
0.6 | 173/76.4 | 610/78.0 | 0.931 | 0.047 |
| Expressed | GCTAAGAACGCTGGACCTAATG/TGGGTGTGCCTTTCTGAATG | 6th 7th |
0.2 | 183/76.0 | 285/76.80 | 0.874 | 0.037 |
* Predicted from tomato cDNA sequences; ** Estimated by agarose gel electrophoresis; *** Mean of 3 technical replicas; N/A = Non-amplification