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. Author manuscript; available in PMC: 2009 Jan 21.
Published in final edited form as: Neuron. 2007 Mar 1;53(5):639–647. doi: 10.1016/j.neuron.2007.01.033

Figure 3. More Examples of Transcomplemented Tracing.

Figure 3

(A–C) Long-range viral spread from a single initially infected cell. (A) A huge cluster of green cells surrounds a single red/green deep-layer cortical neuron (dotted line) at 8 days postinfection. Another dense cluster of cells is also infected in the superficial cortical layers immediately above it, consistent with known projections of superficial layers to deeper ones; distant deep-layer pyramidal cells are also infected, again consistent with known patterns of long-range intralaminar connectivity. To the left of the putatively initially infected cell is a second yellow (double-labeled) cell, apparently secondarily—and recently—infected because of the lack of green cells surrounding it. (B–C) Closeup of central cluster from (A). (D–F) More examples of in situ complementation: clusters of infected cells surrounding isolated putatively postsynaptic ones. Scale bars: 200 μm.