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. Author manuscript; available in PMC: 2009 Jan 22.
Published in final edited form as: J Immunol. 2007 Nov 1;179(9):5936–5946. doi: 10.4049/jimmunol.179.9.5936

FIGURE 8.

FIGURE 8

CD4+CD25+ depletion of CD80-stimulated splenocytes restores the ability to cause diabetes. A, Splenocytes from 6-wk-old NOD mice were adoptively transferred into NOD.scid-RIP-CD80 and NOD.scid-RIP-CD86 mice. When the mice became diabetic, the splenocytes were harvested and stained for CD4+CD25+ and FoxP3+ cells. Top panels, Representative cells from NOD.scid-RIP-CD80 mice; lower panels, cells from NOD.scid-RIP-CD86 mice. B, The splenocytes were depleted of CD4+CD25+ T cells (>80% depleted) and then adoptively transferred to NOD.scid mice which were observed for diabetes. These were compared with cells that had not been depleted of CD4+CD25+ cells. The results shown for the transfer from diabetic NOD.scid-RIP-CD80 mice are combined from two separate experiments.