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. Author manuscript; available in PMC: 2009 Jan 22.
Published in final edited form as: J Am Chem Soc. 2007 Nov 28;129(50):15428–15429. doi: 10.1021/ja0761784

Figure 1.

Figure 1

Alanine labeling and 1H-13C 2D Methyl TROSY spectra of (A) 13CH3-Ala,U-2H labeled Ufd1in UN (B) 13CH3-Ala,U-2H labeled p97 ND1 (top) and 13CδH3-Ile, 13CH3-Ala,U-2H labeled p97ND1 (bottom) (C) Expanded region of the 1H-13C 2D Methyl TROSY spectrum of 13CH3-Ala,U-2H labeled p97 ND1 in the absence and presence of perdeuterated Npl4 UBD. (D) Structural model of the p97 ND1-Npl4 UBD complex. The p97 hexamer is shown as a grey ribbon and perturbed alanine residues are indicated by red (most shifted), orange (intermediate) and yellow (least) balls. The position of Npl4 UBD (blue surface) in complex with p97 N domain is shown for one of monomers. Details are given in supplementary information.