Fig. 2. The N-domain of CEACAMs is important for Dr adhesin binding.

A) Sequence alignments for N domains of CEACAMs. B) CEA binding assay with E.coli expressing DraE and AfaE-III. Bacterial cells were labeled with ³H-thymidine and incubated with or without the competitor in microtiter wells coated with soluble CEA at a concentration of 5 mg/ml. Competitors were added at final concentrations of 20 μM N-CEA, 80 μM DAF, and 10 mM Cm. Bound bacteria remaining in the wells were quantified by scintillation counting. Results shown are the average of three independent experiments with duplicate wells of each sample. Error bars indicate standard deviation.