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. 2009 Feb 3;4(2):e4309. doi: 10.1371/journal.pone.0004309

Figure 1. 7DHC metabolism by rat adrenal glands.

Figure 1

Finely cut fragments of rat adrenal glands were incubated in the presence (B–D) or absence (A) of 7DHC (0.5 mM). Boiled adrenal fragments (C) served as an additional negative control. Panel B shows results from an incubation which included trilostane, a 3βHSD inhibitor. Steroids were extracted with methylene chloride and subjected to LC/MC and UV spectra analyses. Panels A–D identifies 7 main products. Product 3 had an identical retention time to 7DHP standard. Panel E shows UV spectra, MS and the predicted structures of the 7 metabolites.