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. 2009 Feb 3;4(2):e4309. doi: 10.1371/journal.pone.0004309

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Slominski et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fragments of pig adrenal glands were incubated with 0.5 mM 7DHC. Steroids were extracted with methylene chloride and subjected to HPLC and UV spectral analyses. The UV spectrum for all peaks is the same (see arrows). The mass spectra of the collected peaks 1–4 were obtained by direct injection into the MS module of the Bruker Esquire-LC/MS Spectrometer.