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. 2009 Feb 3;4(2):e4309. doi: 10.1371/journal.pone.0004309

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Slominski et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fragments of the pig adrenal glands were incubated with 7DHP (S; 0.5 mM) in the absence (A) or presence (B) of trilostane (0.1 mM) and analyzed by RP-HPLC. Boiled adrenal fragments were used as a negative control (blue). The major products of 7DHP metabolism affected by trilostane are marked by numbers. Time-dependent changes in the relative concentration of the identified products in presence (T+) or absence (T-) of trilostane were calculated using ACDLabs software and are presented in C.