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. 2009 Feb 3;4(2):e4309. doi: 10.1371/journal.pone.0004309

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Slominski et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. To study 7DHC transfer between membranes by N-62 StAR protein cholesterol or 7DHC was placed in donor vesicles at a molar ratio to phospholipid of 0.2. The transfer of the sterol to acceptor vesicles at 35°C was measured in the presence or absence of 5 µM N-62 StAR protein. B. Inhibition of the side chain cleavage of [4-¹⁴C]cholesterol by 7DHC was determined with substrate and cytochrome P450scc incorporated into phospholipid (PL) vesicles prepared from dioleoyl phosphatidylcholine containing ardiolipin. As indicated in the figure, 7DHC was included in the membrane at a molar ratio to phospholipid of 0.2. The rate of side chain cleavage of [4-¹⁴C]cholesterol was determined from the amount of [4-¹⁴C]pregnenolone formed.