Table 2.
Back-calculated concentrations from calibration curves run in duplicate on six occasions
| Nominal (ng/mL) |
GM (ng/mL) |
S.D. (ng/mL) |
DEV(%) |
R.S.D.(%) |
n |
|---|---|---|---|---|---|
| 1.00 | 0.97 | 0.041 | -3.34 | 4.27 | 12 |
| 2.00 | 1.98 | 0.149 | -0.78 | 7.49 | 12 |
| 5.00 | 4.93 | 0.228 | -1.37 | 4.62 | 12 |
| 10.0 | 10.34 | 0.470 | 3.36 | 4.55 | 12 |
| 25.0 | 25.41 | 0.950 | 1.63 | 3.74 | 12 |
| 50.0 | 51.12 | 1.842 | 2.25 | 3.60 | 12 |
| 100 | 98.25 | 2.266 | -1.75 | 2.31 | 12 |
Abbreviations: GM, grand mean; S.D., standard deviation; DEV, percent deviation from nominal value; R.S.D., relative standard deviation; n, total number of replicate observations within the validation runs.