Table 4.

Assessment of recovery and matrix effect.

Sample concentration	Peak Area				Extraction Efficiencye	Matrix Effectf	Drying Lossg	Absolute Recoveryh
	Aa	Bb	Cc	Dd
5 ng/ml	47119.5	50466.8	53537.1	54282.8	93.4%	5.7%	1.4%	86.8%
75 ng/ml	735281.4	812394.8	805937.2	819856.8	90.5%	-0.8%	1.7%	89.7%

Experiments were conducted in five replicates for each different sample condition:

^aFinasteride spiked into blank plasma, extracted, dried and reconstituted

^bFinasteride spiked into supernatant from blank plasma extraction, dried and reconstituted

^cFinasteride spiked into ethyl acetate, dried and reconstituted

^dFinasteride spiked into mobile phase directly

^eExtraction Efficiency =A/B*100%

^fMatrix Effect =(1-B/C)*100%

^gDrying Loss =(1- C/D)*100%

^hAbsolute Recovery = A/D*100%