Figure 4.

EBP1 overexpression inhibits the response to HRG. A, EBP1 and vector control transfected cells were plated in complete medium at 5 × 10³ per well for 1 d. The next day, cells were switched to steroid-reduced medium and HRG was added at the indicated concentrations. Total cell numbers were determined 7 d later by MTT assay. Points, mean of six wells; bars, SE. Representative of three experiments. B, AKT activation in response to HRG C81-EBP1 or vector control cells were serum starved overnight and then treated with HRG (20 ng/mL) for the indicated times (min). Cell lysates were immunoblotted with antibody to phosphorylated AKT or total AKT as indicated. C, densitometric evaluation of AKT phosphorylation. Representative of two independent experiments.