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. Author manuscript; available in PMC: 2009 Jan 22.
Published in final edited form as: Insect Mol Biol. 2003 Oct;12(5):483–490. doi: 10.1046/j.1365-2583.2003.00433.x

Table 1.

Kinetic parameters of rAeKMO*

Substrates/cofactors Km (mM) Vmax (nmol/min/mg) Kcat (/min) Kcat/Km (min/mM)
Kynurenine 0.89 ± 0.07 3550 ± 87 113 ± 2.8 127 ± 12
NADPH 0.82 ± 0.10 3821 ± 188 122 ± 6.0 149 ± 26
NADH 5.17 ± 0.97 1616 ± 110 51 ± 3.5 10 ± 2.6
*

The biochemical activities were measured as described in the Experimental procedures. The kinetic parameters of rAeKMO were calculated by fitting the experimental data to the Michaelis–Menten equation using the Enzyme Kinetics Module (SPSS Science).