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. Author manuscript; available in PMC: 2009 Jan 22.
Published in final edited form as: Plant J. 2007 Jun 15;51(5):840–849. doi: 10.1111/j.1365-313X.2007.03181.x

Figure 5.

Figure 5

miR172 and SUP mRNA levels in various genotypes.

(a) miR172 levels in inflorescences of wild-type and ag-1 plants as determined by RNA filter hybridization. The region of the gel where tRNAs migrate is shown below the hybridization image to indicate the amount of RNAs loaded.

(b) SUP RNA levels in inflorescences of wild-type and 35S::AP2m3 plants as determined by RT-PCR. UBQ RNA serves as an internal control. Thirty-five and 25 cycles of PCR are shown for SUP and UBQ, respectively, although shorter and longer cycles were performed for each gene to ensure that these numbers of cycles did not result in saturation of the reactions. -RT, no reverse transcription.