Table 3.

Comparison of mutant allele frequencies estimated by subcloning and RSI^*

				Mutant Allele Frequency
Tumor ID	Exon	Sequencing of Tumor DNA†	Sequencing of Clones	RSI (%)*	Cloning‡	(%)
4_1	3	763 deletion	763 delCTCTACG	20	18/56	32
4_6	2	613 G>T	613 G>T	40	17/48	35
4_8	2	652 A>G	652 A>G	50	21/38	55
4_12	1	469 C>T	469 C>T	40	9/31	29
4_16	2	581 deletion	581 deletion	30	21/58	36
4_31	3	IVS2−1 G>A	IVS2−1 G>A	25	3/40	8
4_47	3	752 deletion	752 delTCGTCA	30	18/54	33
4_53	2	574 insertion	574 delGA	10	20/57	35
4_65	2	570 deletion	570 delCAGAGAT	20	24/55	44
4_70	2	665 T>C	665 T>C	30	21/50	42

^*Relative Signal Intensity (RSI) of mutant allele compare with total fluorescence

^†Insertions and deletions were not characterized in tumor DNA sequencing

^‡Number of positive transformants