Skip to main content
. 2009 Jan 19;3:1. doi: 10.3389/neuro.05.001.2009

Figure 5.

Figure 5

Transsynaptic double labeling of neurons in the hippocampus after 6 days following bilateral injections of two RV-vectors into MEC. (A) Schematic representation of the relevant circuitry in the hippocampal formation. The colored panels show the injection site (green: rHEP5.0-CVSG-EGFPx2, magenta: rHEP5.0-CVSG-β-gal). (B) Fluorescence micrograph showing the distribution of labeled neurons in the dorsal hippocampus. In field CA1, neurons are labeled only by the virus vector injected into the ipsilateral MEC, whereas β-gal (magenta) and GFP (green) labels intermingle in CA3 region. Note that double-labeled neurons are observed in CA3. (C–E) Labeled CA3 pyramidal cells taken from the box in (B). Neurons labeled by both β-gal (C) and GFP (D) can be seen in white in the merged image (E). Arrows indicate double-labeled neurons. (F–H) Photomicrographs demonstrating Golgi-like labeling of a double-labeled CA3 pyramidal cell. High magnification image shows that the dendrites and their spines can also be visualized in the double-labeled neuron. MEC, medial entorhinal cortex. Scale bar = 500 μm in (B), 50 μm in (E) [applies to (C,D)], 20 μm in (H) [applies to (F,G)], 10 μm in (I).