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. 2009 Jan 27;4(1):e4295. doi: 10.1371/journal.pone.0004295

Figure 1. PIK3CA overexpression was an early genetic event during ovarian tumorigenesis.

Figure 1

A. PIK3CA mRNA expression was significantly up-regulated in the established epithelial ovarian cancer cell lines (n = 15) compared with primary cultured ovarian surface epithelial cells (n = 6, p = 0.041). mRNA expression was measured by real-time RT-PCR. B. mRNA expression level of PIK3CA was significantly upregulated in ovarian cancer specimens compared with normal control ovarian epithelium. Normal ovarian epithelium was isolated by laser-capture microdissection. There was no further significant increase of PIK3CA mRNA expression after malignant transformation among different stages of ovarian cancer.