Table 2. Primers used for evaluating the variations by heteroduplex-single strand conformational polymorphism analysis.
| Variations | Primer sequence (5'-3') | Size of PCR product (bp) | Annealing temp (°C) |
|---|---|---|---|
| c.192C>G |
F-TGCCCTCAGCCCTCAAGTATC |
340 |
66 |
| R-CAGCCCAAGCAGCAGGAGGAG |
|||
| c.55-14_55-13insGTAT |
F-CTTTTCCCTCGGGTAGTAGA |
344 |
62 |
| R-GACGCTGTAGCTGGCATCCT |
|||
| c.664C>A |
F-TCTCTGGCTGACCCTGCTCTT |
152 |
60 |
| c.669G>A |
R-AAACCAAATCCTTCCACACTGCT |
||
| c.770G>A | F-TTGGGGGTTGAGAAAATAGG |
283 |
60 |
| R-TGGGTGGAGGGGAAGAAAGTG |
In the "Primer sequence" column, "F" indicates the forward sequence and "R" indicates the reverse sequence.