. Author manuscript; available in PMC: 2009 Apr 15.

Published in final edited form as: J Immunol. 2008 Apr 15;180(8):5187–5200. doi: 10.4049/jimmunol.180.8.5187

Table III.

Apoptosis of Tg DO11.10 CD4⁺ T cells

Treatment ^a		% Apoptosis of DO11.10 Tg CD4⁺ T Cells ^b
Ag	Dose	24 hours ^c	P₁^d	72 hours ^e	P₂^f
media		7.06 ± 0.6		10.37 ± 0.92
OVA	1 mg/ml	10.96 ± 2.82		12.73 ± 6.53
OVA-pσ1	1 mg/ml	67.35 ± 7.97	< 0.001	83.80 ± 7.10	< 0.001
OVA-pσ1	0.05 mg/ml	30.99 ± 3.98	< 0.001	54.41 ± 11.6	0.003
pσ1	1 mg/ml	77.82 ± 4.82	< 0.001	86.52 ± 11.8	< 0.001
pσ1	0.05 mg/ml	32.43 ± 7.26	0.005	62.58 ± 10.6	< 0.001
OVA + pσ1	1 mg/ml, 1 mg/ml	77.56 ± 8.42	< 0.001	80.34 ± 8.44	< 0.001
OVA + pσ1	1mg/ml, 0.05 mg/ml	29.83 ± 9.86	0.025	62.29 ± 10.13	< 0.001

Lymphocytes were isolated from spleens of naïve DO11.10 Tg mice. Cells were stimulated in vitro with designated concentrations of Ags and analyzed by FACS.

Apoptosis was determined by co-staining with Annexin-V and 7-AAD.

^{c, e}

Mean ± SD of three to four replicates are presented after 24 and 72 h of culture.

^{d, f}

p-values for DO11.10 CD4⁺ T cells stimulated with OVA versus OVA-pσ1, pσ1, or OVA + pσ1 for 24 or 72 hours.