. Author manuscript; available in PMC: 2009 Apr 15.

Published in final edited form as: J Immunol. 2008 Apr 15;180(8):5187–5200. doi: 10.4049/jimmunol.180.8.5187

Table IV.

Cytokine production by in vitro cultured DO11.10 lymphocytes

Treatment ^a		Cytokine Secretion after 24 Hours ^b
Ag	Dose	IL-4 (ng/ml) ^d	IL-10 (ng/ml) ^e	IL-17 (ng/ml) ^f	IFN-γ (ng/ml) ^g
OVA	1 mg/ml	0.58 ± 0.03	0.66 ± 0.04	5.59 ± 0.54	4.06 ± 0.52
OVA-pσ1	1 mg/ml	1.20 ± 0.1 ^§	2.76 ± 0.14 ^*	0.37 ± 0.11 ^*	0.31 ± 0.08 ^§
OVA-pσ1	0.05 mg/ml	1.67 ± 0.2 ^§	4.73 ± 0.29 ^*	0 ± 0 ^*	0 ± 0 ^*
pσ1	1 mg/ml	0 ± 0 ^*	0.26 ± 0.04 ^§	0.63 ± 0.17 ^*	0.22 ± 0.07 ^§
pσ1	0.05 mg/ml	0 ± 0 ^*	0 ± 0 ^*	0.58 ± 0.14 ^*	0 ± 0 ^*
OVA + pσ1	1 mg/ml, 1 mg/ml	0 ± 0 ^*	0.56 ± 0.02	4.17 ± 0.6	2.6 ± 0.45
OVA + pσ1	1mg/ml, 0.05 mg/ml	0 ± 0 ^*	0.42 ± 0.17	1 ± 0.14 ^§	0.97 ± 0.08 ^§

		Cytokine Secretion after 72 Hours ^c
OVA	1 mg/ml	2.21 ± 0.13	1.82 ± 0.12	6.22 ± 0.46	10.1 ± 0.98
OVA-pσ1	1 mg/ml)	0 ± 0 ^*	0 ± 0 ^*	0 ± 0 ^*	0 ± 0 ^*
OVA-pσ1	0.05 mg/ml	3.54 ± 0.15 ^§	2.2 ± 0.03 ^§	0.52 ± 0.13 ^*	0.38 ± 0.03 ^*
pσ1	1 mg/ml	0 ± 0 ^*	0 ± 0 ^*	0.58 ± 0.14 ^*	0.72 ± 0.05 ^*
pσ1	0.05 mg/ml	0 ± 0 ^*	0.29 ± 0.02 ^*	0 ± 0 ^*	0.94 ± 0.09 ^*
OVA + pσ1	1 mg/ml, 1 mg/ml	0 ± 0 ^*	0.37 ± 0.19 ^§	0.72 ± 0.18 ^*	0.77 ± 0.2 ^*
OVA + pσ1	1mg/ml, 0.05 mg/ml	1.22 ± 0.18 ^*	0.46 ± 0.16 ^§	2.87 ± 0.47 ^§	2.3 ± 0.29 ^§

Lymphocytes were isolated from spleens of naïve DO11.10 Tg mice.

Cultured supernatants from cells stimulated in vitro with designated concentrations of Ags were analyzed by cytokine ELISA.

^{b, c}

cells were cultured with or without Ag for 24 or 72 hours.

^d-g

Cytokine concentration was measured in triplicates and is depicted as an average ± SEM. Presented cytokine concentration is corrected over the cytokine production by unstimulated cells.

Statistical significance was calculated by Student’s t test;

p ≤ 0.001

^§

p < 0.05 for the differences between cells stimulated with OVA vs. OVA-pσ1, pσ1 or OVA + pσ1 - stimulated cells, for each time point.