Table 1.
Primers for real time PCR, amplicon size, values of slope, PCR efficiency and linearity (R2).
| Genes | Primers | 5' → 3' | Amplicon size (bp) | Slope | PCR Efficiency | R2 |
| GNA15 | Forward | GAGAACCGCATGAAGGAGAG | 84 | -3.312 | 100.0 | 0.991 |
| Reverse | AAAGAGGATGACGGATGTGC | |||||
| KRT31 | Forward | TGAGCAGGAGGTCAATACCC | 110 | -2.913 | 120.4 | 0.990 |
| Reverse | GACTCCTGGTCTCGTTCAGC | |||||
| BST2 | Forward | GGAGGAGCTTGAGGGAGAG | 75 | -3.476 | 93.9 | 0.991 |
| Reverse | CTCAGTCGCTCCACCTCTG | |||||
| MFAP2 | Forward | GCCGTGAGGAACAGTACCC | 91 | -3.152 | 107.6 | 0.990 |
| Reverse | CGGAGGCTGTAGAAGCAGAC | |||||
| TUBA6 | Forward | TCAACACCTTCTTCAGTGAAACG | 101 | -3.341 | 99.2 | 0.991 |
| Reverse | AGTGCCAGTGCGAACTTCATC | |||||
| GAPDH | Forward | ACCCACTCCTCCACCTTTGA | 101 | -3.392 | 97.1 | 0.990 |
| Reverse | CTGTTGCTGTAGCCAAATTCGT | |||||
| ACTB | Forward | GGCACCCAGCACAATGAAG | 66 | -3.255 | 102.8 | 0.994 |
| Reverse | CCGATCCACACGGAGTACTTG | |||||