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. Author manuscript; available in PMC: 2009 Jan 22.
Published in final edited form as: J Am Chem Soc. 2007 May 2;129(20):6619–6625. doi: 10.1021/ja071013g

Figure 2.

Figure 2

Phage display selection scheme. The AP2 library (blue) is fused to the pIII coat protein. B represents biotin. After phage production in E. coli strain ER2738, a fraction of the phage pool is pre-biotinylated by endogenous BirA. In step 1 of the selection, streptavidin-coated beads are used to remove these pre-biotinylated phages (negative selection). In step 2, the remaining phage are treated with the BL2 enzyme. In step 3, streptavidin-coated beads are used to isolate the biotinylated phage from this mixture (positive selection). Finally (step 4), the recovered biotinylated phage are amplified in ER2738 for another round of selection.