Figure 2. Three-dimensional reconstructions of adult rescued swr inner ears.

(A–F) Three-dimensional reconstructions of the inner ears of two adult rescued swr fish (swirl 1 (A, D) and swirl 2 (B, E)) and an age-matched wild-type fish (C, F). Semicircular canals are absent in the rescued swr fish ears, while the ampullae, crus commune, utricle, saccule and lagena are present and appear normal. Note that the pars superior (ampullae, crus commune and utricle) of swirl 1 is twisted relative to the pars inferior (saccule and lagena), about the point marked with the arrow (we are unable to tell whether or not this is an experimental artefact). The kink in the lateral canal of the wild-type ear (C) is an artefact. The asterisk marks the transverse canal that links the two saccules across the midline. (G, H) Sketch of the inner ears of adult rescued swr and wild-type fish. Shading in the wild-type ear indicates the regions missing from rescued swr ears. Abbrevations: a-amp, anterior ampulla; l-amp, lateral ampulla; p-amp, posterior ampulla; a-canal, anterior semicircular canal; l-canal, lateral semicircular canal; p-canal, posterior semicircular canal; cc, crus commune. swirl 1 is a smad5-rescued swr^dc24 fish; swirl 2 is a bmp2b-rescued swr^ta72 fish. A–C, G and H are lateral views of left hand ears, with anterior to the left; D–F are dorsal views. Scale bar, 500 µm.