Figure 2. Plate based survival assay.
A Graph showing the percentage survival of WT and mutant variants of SH1000 when serial dilutions of the strains were plated on BHI agar containing 1 mM linoleic acid. Survival is expressed as a percentage of viable cell counts obtained for control plates lacking linoleic acid. Values are the mean of multiple independent experiments. Error bars indicate standard errors of the mean. p<0.005 for each mutant by Student's t-test. B Plates showing the relative survival of SH1000 and the sasF (Liv694) and vraE (Liv753) mutants on BHI agar containing 0 or 1 mM linoleic acid. The 10−1 to 10−6 dilution series of cultures are as indicated.