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. Author manuscript; available in PMC: 2009 Jan 23.
Published in final edited form as: J Infect Dis. 2006 Apr 12;193(10):1464–1470. doi: 10.1086/503747

Figure 3.

Figure 3

Western blot of tissue extracts of jejunal biopsies probed with anti-HD5 antibody. Gel electrophoresis was performed by acid-urea polyacrylamide gel electrophoresis in which peptides are separated by both size and charge. Recombinant peptides are therefore used as markers of migration. Lane 1, recombinant pro-HD5 (20-94); lane 2, recombinant HD5; lanes 3-7, individual biopsies; lane 8, control ileal tissue from healthy patient. The quantity and isoform distribution of propeptide shows little variation. The diffuse, slow migrating band above the pro-HD5 control corresponds to an O-linked glycan modification of the HD5 (20-94) propeptide (C. L. Bevins, unpublished data).