Figure 2. Expression pattern of EAc-enriched genes by in situ hybridization on sagittal brain sections.

Dig-labeled RNA in situ hybridization (ISH) was performed on 25μm sagittal adult mouse brain sections 49 genes whose specific expression pattern in EAc network has not been reported earlier. In this figure, examples of low expressed genes (A), strong and ubiquitously expressed genes (B) and potential novel EAc markers (C) are shown. Neuropeptide Y (Npy) was used as a low intensity positive control, preproenkephalin (Penk) was used as a high intensity positive control and a blank hybridization was used as the negative control (D). Representative images are shown, and the complete ISH analysis on sagittal sections for all 49 genes is shown in supplemental Figure S2. Expression patterns were classified as detailed in methods. Gene symbols are indicated as in Table 1.