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. Author manuscript; available in PMC: 2009 Mar 30.
Published in final edited form as: Virology. 2008 Feb 20;373(1):98–111. doi: 10.1016/j.virol.2007.11.012

Figure 2.

Figure 2

Nectin-1 expression on B78H1-C10 cells after co-cultivation with gD expressing cells. Nectin-1 positive B78H1-C10 cells were mixed at an equal ratio with B78-gD(A3C-Y38C) cells (left), B78-gDwt (center) or B78-gD(W294A) cells (right). After 16 h of co-culture, cultures were fixed, permeabilized and stained. Nectin-1 on target B78H1-C10 cells was detected with mouse monoclonal antibody CK41 followed by anti-mouse Ig coupled to Alexa488 shown in green (D,E,F). gD on effector cells was detected with rabbit polyclonal serum R7 followed by anti-rabbit Ig coupled with Alexa594 shown in red (G,H,I). A phase contrast image was taken to show all cells in the co-cultures (A,B,C) and DAPI was used to stain all nuclei (blue) in merged images (J,K,L). Arrows point to target cells not directly in contact with effector cells, thus retaining nectin-1 staining. A representative experiment is shown. (M) Histograms showing that gD expression on effectors cells is not affected after 16 hr co-culture with B78H1-C10 (gray) compared to B78H1 (black line) target cells. gD on effector cells (Qdot655 negative) was detected using mAb MC5 followed by anti-mouse Ig coupled to Alexa488.