Abstract
Hepatitis C viruses (HCV) present in 110 Italian patients were characterized by genotype-specific PCRs. Among the 65 cases of community-acquired hepatitis, HCV genotype II was dominant (60%), followed by genotypes IV (15%), III (11%), and I (3%). Among the 45 hemophilia-associated cases, the distribution of the four HCV genotypes was markedly different: genotype I was the most prevalent (61%), followed by genotypes II (25%), III (4%), and IV (2%). Double infections were observed in eight patients. Two HCV remained unclassified. For the 45 community-acquired cases from which a liver biopsy was available, genotype II was associated with more severe liver damage than the other types.
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- Bréchot C., Kremsdorf D. Genetic variation of the hepatitis C virus (HCV) genome: random events or a clinically relevant issue? J Hepatol. 1993 Mar;17(3):265–268. doi: 10.1016/s0168-8278(05)80203-x. [DOI] [PubMed] [Google Scholar]
- Cha T. A., Beall E., Irvine B., Kolberg J., Chien D., Kuo G., Urdea M. S. At least five related, but distinct, hepatitis C viral genotypes exist. Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):7144–7148. doi: 10.1073/pnas.89.15.7144. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chan S. W., McOmish F., Holmes E. C., Dow B., Peutherer J. F., Follett E., Yap P. L., Simmonds P. Analysis of a new hepatitis C virus type and its phylogenetic relationship to existing variants. J Gen Virol. 1992 May;73(Pt 5):1131–1141. doi: 10.1099/0022-1317-73-5-1131. [DOI] [PubMed] [Google Scholar]
- Choo Q. L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science. 1989 Apr 21;244(4902):359–362. doi: 10.1126/science.2523562. [DOI] [PubMed] [Google Scholar]
- Houghton M., Weiner A., Han J., Kuo G., Choo Q. L. Molecular biology of the hepatitis C viruses: implications for diagnosis, development and control of viral disease. Hepatology. 1991 Aug;14(2):381–388. [PubMed] [Google Scholar]
- Imberti L., Cariani E., Bettinardi A., Zonaro A., Albertini A., Primi D. An immunoassay for specific amplified HCV sequences. J Virol Methods. 1991 Oct;34(3):233–243. doi: 10.1016/0166-0934(91)90103-7. [DOI] [PubMed] [Google Scholar]
- Okamoto H., Sugiyama Y., Okada S., Kurai K., Akahane Y., Sugai Y., Tanaka T., Sato K., Tsuda F., Miyakawa Y. Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infectious sources. J Gen Virol. 1992 Mar;73(Pt 3):673–679. doi: 10.1099/0022-1317-73-3-673. [DOI] [PubMed] [Google Scholar]
- Pistello M., Ceccherini-Nelli L., Cecconi N., Bendinelli M., Panicucci F. Hepatitis C virus seroprevalence in Italian haemophiliacs injected with virus-inactivated concentrates: five year follow-up and correlation with antibodies to other viruses. J Med Virol. 1991 Jan;33(1):43–46. doi: 10.1002/jmv.1890330109. [DOI] [PubMed] [Google Scholar]
- Ravaggi A., Primi D., Cariani E. Direct PCR amplification of HCV RNA from human serum. PCR Methods Appl. 1992 May;1(4):291–292. doi: 10.1101/gr.1.4.291. [DOI] [PubMed] [Google Scholar]
- Takada N., Takase S., Takada A., Date T. Differences in the hepatitis C virus genotypes in different countries. J Hepatol. 1993 Mar;17(3):277–283. doi: 10.1016/s0168-8278(05)80205-3. [DOI] [PubMed] [Google Scholar]
- Yoshioka K., Kakumu S., Wakita T., Ishikawa T., Itoh Y., Takayanagi M., Higashi Y., Shibata M., Morishima T. Detection of hepatitis C virus by polymerase chain reaction and response to interferon-alpha therapy: relationship to genotypes of hepatitis C virus. Hepatology. 1992 Aug;16(2):293–299. doi: 10.1002/hep.1840160203. [DOI] [PubMed] [Google Scholar]