Table 2.
Plasmid (promoter) | C & N source | Specific activities, nmol/min per mg* |
---|---|---|
pCR1 (dauB) | Glu | 34 |
Glu + d-arg | 680 | |
Glu + l-arg | 63 | |
pZY4 (dauT) (7) | Glu | 619 |
Glu + d-arg | 6006 | |
Glu + l-arg | 233 |
C & N, carbon and nitrogen; Glu, cells were grown in MMP-glutamate; Glu + d-arg, cells were grown in MMP-glutamate supplemented with 5 mM d-arginine; and Glu + l-arg, cells were grown in MMP-glutamate supplemented with 20 mM l-arginine.
*Specific activities of β-galactosidase expressed from plasmids were averages of 2 measurements for each growth condition, with standard errors of less than 5%.