Table 5.

Effect of d- or l-arginine on the aruC promoter activity in PAO1 and its mutants

Strain (genotype)	C & N source	Specific activities, nmol/min per mg*	Fold change
PAO1 (wild type)	Glu	501	1.0
	Glu + d-arg	7007	14.0
	Glu + l-arg	11104	22.2
PAO5801 (dauB)	Glu	349	1.0
	Glu + d-arg	750	2.2
	Glu + l-arg	6950	19.9
PAO5802 (dauA)	Glu	380	1.0
	Glu + d-arg	610	1.6
	Glu + l-arg	7345	19.3

Glu, cells were grown in glutamate minimal medium; Glu + d-arg, cells were grown in glutamate minimal medium supplemented with 5 mM d-arginine; Glu + l-arg, cells were grown in glutamate minimal medium supplemented with 20 mM l-arginine.

*Specific activities of β-galactosidase expressed from the aruC::lacZ fusion plasmid pSC500 [Park SM, Lu CD, Abdelal AT (1997) J Bacteriol 79:5309–5317] were averages of 2 measurements for each growth condition, with standard errors of less than 5%.