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. 2009 Jan 7;106(3):814–819. doi: 10.1073/pnas.0807583106

Fig. 4.

Fig. 4.

Etsrp71 transcriptionally activates the Tie2 gene. (A) Tie2-lacZ Tg and Etsrp71± mice were mated to analyze β-galactosidase expression in WT (+/+) and Etsrp71 null (−/−) littermate embryos at the indicated developmental stages. Note the absence of β-galactosidase expression in the Etsrp71 null embryos. (B) Etsrp71 binds to the upstream promoter fragment of the Tie2 gene. Promoter occupancy of Etsrp71 in the upstream promoter fragment (UPF) and enhancer modules of the Tie2 gene was evaluated using a ChIP assay. Note the Etsrp71 binding to the UPF only (not the intronic enhancer of the Tie2 gene). (C) Etsrp71 transactivates Tie2 expression from UPF. Schematic of a 2.1-kb UPF fused to the luciferase (Luc) reporter is shown. Transcriptional assays in C2C12 myoblast cells reveal a dose-dependent induction of luc activity by Etsrp71 (black). Mutation of the Ets-binding site completely abolished the transcriptional activity (hatched) (*, P < 0.001). Each assay was analyzed in triplicate and repeated twice.