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. 2009 Jan 7;106(3):929–934. doi: 10.1073/pnas.0811683106

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© 2009 by The National Academy of Sciences of the USA

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Fig. 5. — Bioassays demonstrating the synergistic action of the R. fascians CK bases. Equimolar mixes or calculated additive effect of iP, 2MeSiP, cZ, and 2MeScZ (eq4, add4), and of iP, 2MeSiP, cZ, 2MeScZ, tZ, and 2MeStZ (eq6, add6). (A) Histochemical staining of the AHK4:GUS reporter line treated with 10 μM of the individual CKs or of the equimolar mixes for 10 days. (B) Loss of chlorophyll content and anthocyanin accumulation upon CK treatment. The chlorophyll content and anthocyanin accumulation are represented relative to untreated (control) plants and to eq6 treatment, respectively. Individual CKs, 10 μM; add4, average number of events provoked by the 4 CKs at 1 μM as a measure for the additive effect of these CKs; eq4, number of events provoked by the equimolar mix at a final concentration of 10 μM (containing 2.5 μM of each CK); add6 represents the average number of events provoked by the 6 CKs at 0.1 μM as a measure for the additive effect of these CKs; eq6, number of events provoked by the equimolar mix at a final concentration of 1 μM (containing 0.167 μM of each CK). (C) Root inhibition, and callus and shoot induction events observed in the regeneration assays; add4, average number of events provoked by the 4 CKs at 1 μM as a measure for the additive effect of these CKs; eq4, number of events provoked by the equimolar mix at a final concentration of 10 μM (containing 2.5 μM of each CK). (D) Root inhibition, and callus and shoot induction events observed in the regeneration assays; add6 represents the average number of events provoked by the 6 CKs at 0.1 μM as a measure for the additive effect of these CKs; eq6, number of events provoked by the equimolar mix at a final concentration of 1 μM (containing 0.167 μM of each CK). (E) Deetiolation of Arabidopsis plants on CK treatment. The number of events (closed or open cotyledons, and leaf formation) is given as percentages of the total number of germinated seedlings. Individual CKs, number of events scored at 1 μM; add6, sum of events scored for the 6 CKs at 1 μM; eq6, number of events scored for the equimolar mix at a final concentration of 6 μM (containing 1 μM of each CK). (F) Dose–response curve of the CK-dependent tobacco callus growth. The growth of untreated (control) callus is represented by the dotted line. Error bars represent SDs (n = 6).