Fig. 4.

P_minor spacer affects the conformation of P_minor DNA. (A) Mobilities of P_min derivatives with different spacer sequences and P_lacUV5-Mut (5′-³²P end-labeled 51-bp fragments) upon electrophoresis in a native, 12% polyacrylamide gel. (B) Predicted structures of P_min, P_min/GC, P_min/comp, and P_lacUV5-Mut DNA. All structures were aligned using the common sequence GAATTC at the 5′ end. In addition, P_min, P_min/GC, and P_min/comp have identical sequences except for their 14-bp spacer sequences (Fig. 1B).