Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Jan;32(1):249–252. doi: 10.1128/jcm.32.1.249-252.1994

A novel kit for rapid detection of Vibrio cholerae O1.

J A Hasan 1, A Huq 1, M L Tamplin 1, R J Siebeling 1, R R Colwell 1
PMCID: PMC263010  PMID: 8126193

Abstract

We report on the development and testing of a novel, rapid, colorimetric immunodiagnostic kit, Cholera SMART, for direct detection of the presence of Vibrio cholerae O1 in clinical specimens. Unlike conventional culture methods requiring several days to complete, the Cholera SMART kit can be used directly in the field by untrained or minimally skilled personnel to detect V. cholerae O1 in less than 15 min, without cumbersome laboratory equipment. A total of 120 clinical and environmental bacterial strains, including both O1 and non-O1 serotypes of V. cholerae isolated from samples collected from a variety of geographical regions, were tested, and positive reactions were observed only with V. cholerae O1. Also, results of a field trial in Bangladesh, employing Cholera SMART, showed 100% specificity and 96% sensitivity compared with conventional culture methods. Another field trial, in Mexico, showed that Cholera SMART was 100% in agreement with a recently described coagglutination test when 108 stool specimens were tested.

Full text

PDF
249

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams L. B., Siebeling R. J. Production of Vibrio cholerae O1 and non-O1 typing sera in rabbits immunized with polysaccharide-protein carrier conjugates. J Clin Microbiol. 1984 Feb;19(2):181–186. doi: 10.1128/jcm.19.2.181-186.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brayton P. R., Tamplin M. L., Huq A., Colwell R. R. Enumeration of Vibrio cholerae O1 in Bangladesh waters by fluorescent-antibody direct viable count. Appl Environ Microbiol. 1987 Dec;53(12):2862–2865. doi: 10.1128/aem.53.12.2862-2865.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Colwell R. R., Hasan J. A., Huq A., Loomis L., Siebeling R. J., Torres M., Galvez S., Islam S., Tamplin M. T., Bernstein D. Development and evaluation of a rapid, simple, sensitive, monoclonal antibody-based co-agglutination test for direct detection of Vibrio cholerae 01. FEMS Microbiol Lett. 1992 Oct 15;76(3):215–219. doi: 10.1016/0378-1097(92)90339-p. [DOI] [PubMed] [Google Scholar]
  4. Fields P. I., Popovic T., Wachsmuth K., Olsvik O. Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J Clin Microbiol. 1992 Aug;30(8):2118–2121. doi: 10.1128/jcm.30.8.2118-2121.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jesudason M. V., Thangavelu C. P., Lalitha M. K. Rapid screening of fecal samples for Vibrio cholerae by a coagglutination technique. J Clin Microbiol. 1984 May;19(5):712–713. doi: 10.1128/jcm.19.5.712-713.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Koch W. H., Payne W. L., Wentz B. A., Cebula T. A. Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods. Appl Environ Microbiol. 1993 Feb;59(2):556–560. doi: 10.1128/aem.59.2.556-560.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MONSUR K. A. A highly selective gelatin-taurocholate-tellurite medium for the isolation of Vibrio cholerae. Trans R Soc Trop Med Hyg. 1961 Sep;55:440–442. doi: 10.1016/0035-9203(61)90090-6. [DOI] [PubMed] [Google Scholar]
  8. Rahman M., Sack D. A., Mahmood S., Hossain A. Rapid diagnosis of cholera by coagglutination test using 4-h fecal enrichment cultures. J Clin Microbiol. 1987 Nov;25(11):2204–2206. doi: 10.1128/jcm.25.11.2204-2206.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rahman M., Sack D. A., Wadood A., Yasmin M., Latif A. Rapid identification of Vibrio cholerae serotype O1 from primary isolation plates by a coagglutination test. J Med Microbiol. 1989 Jan;28(1):39–41. doi: 10.1099/00222615-28-1-39. [DOI] [PubMed] [Google Scholar]
  10. Sakazaki R., Tamura K., Gomez C. Z., Sen R. Serological studies on the cholera group of vibrios. Jpn J Med Sci Biol. 1970 Feb;23(1):13–20. doi: 10.7883/yoken1952.23.13. [DOI] [PubMed] [Google Scholar]
  11. Shirai H., Nishibuchi M., Ramamurthy T., Bhattacharya S. K., Pal S. C., Takeda Y. Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae. J Clin Microbiol. 1991 Nov;29(11):2517–2521. doi: 10.1128/jcm.29.11.2517-2521.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES