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. Author manuscript; available in PMC: 2009 Nov 29.
Published in final edited form as: Regul Pept. 2008 Jul 9;151(1-3):106–114. doi: 10.1016/j.regpep.2008.07.001

Fig. 2. Bio-PG (100nM) binding is sensitive to protease treatment.

Fig. 2

IEC-6 (top) or CHO-K1 (bottom) epithelial cells were detached from plastic support by either combined Trypsin-EDTA (Trypsin) treatment or EDTA treatment alone. Cells were processed for bio-PG/Streptavidin-PE labeling for 1 hour at 4°C. The stained cells were analyzed by flow cytometry as described in Material and Methods. The controls represent the fluorescent profiles of the same cells pre-incubated with streptavidin-PE reagent alone under the same conditions.