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. Author manuscript; available in PMC: 2009 Nov 29.
Published in final edited form as: Regul Pept. 2008 Jul 9;151(1-3):106–114. doi: 10.1016/j.regpep.2008.07.001

Fig. 3. Specific binding of bio-PG to the IEC-6 intestinal epithelial cell line.

Fig. 3

(A). Competition of bio-PG binding to IEC-6 cells by an excess of unlabeled ligand. Cells were pre-incubated with 100 nM bio-PG alone (grey line) or in presence of 30- fold excess of PG (black heavy line) prior to labeling by phycoerythrin-conjugated streptavidin (Str-PE) followed by flow cytometry analysis. Control labeling by Str-PE alone is shown as a fine black line. (B). The presence of amidated gastrin (G-17) in the binding reaction does not compete for bio-PG binding to IEC-6 cells. Cells were processed as in (A) but G-17 was used instead of PG in competition experiments (heavy black line). (C). Glycine extended gastrin (G-gly) does not compete for bio-PG binding to IEC-6 cells. Cells were processed as in (A) but G-Gly was used instead of PG in competition experiment (heavy black line).