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. Author manuscript; available in PMC: 2009 Nov 29.
Published in final edited form as: Regul Pept. 2008 Jul 9;151(1-3):106–114. doi: 10.1016/j.regpep.2008.07.001

Fig. 8. FACS analysis of bio-PG binding to viable mouse colonic cells isolated from wild type (WT, left panel) and gastrin knockout (Gas-KO, right panel) C57BL/6 mice.

Fig. 8

Colons were dissociated and the resultant single cell suspensions were pre-incubated with 100 nM of bio-PG alone (heavy line) or in the presence of 50-fold excess of unmodified PG (dotted line) followed by incubation with the secondary reagent, Str-PE. Cells stained with Str-PE alone are shown as a thin line. Labeled cells were analyzed with flow cytometry. The mean percentage (and standard deviation) of the bio-PG-interacting (PE-positive) viable cells for three separate experiments, is shown numerically in each panel above the gated subpopulation (straight line). Dead (DAPI-stained) cells were excluded from this analysis by appropriate gating (not shown).