Figure 4.

Inhibition of DTT-induced fibrinogen binding by echistatin or eristostatin. Inhibition of DTT-induced fibrinogen binding to CHO cells expressing wild human αIIbβ3 (•) or human αIIbβ3 containing either the RαIIb W3:4-1/W4:4-1 loops (▴) or an αIIb D²³²H mutation (■) by echistatin or eristostatin. Fibrinogen binding was normalized to binding in the absence of disintegrin and is plotted against the log of the disintegrin concentration. Trend lines were calculated using Microsoft Excel. Data shown are the mean of 3 (echistatin) and 4 (eristostatin) experiments.