Table 1.

Inhibition of fibrinogen binding to recombinant αIIbβ3^* containing a human, rat, or chimeric human/rat α subunit by 1 mM RGDS

	% inhibition†
WT α subunits
Human	85.7 ± 1.2
Rat	23.8 ± 12.7
αIIb W3:4-1 substitutions (rat→human)
ΔV156	83.7 ± 4.4
E157S	60.0 ± 3.6
D159G	45.9 ± 1.4
W162G	60.5 ± 4.6
αIIb W4:4-1 substitutions (rat→human)
E229V	91.1 ± 4.7
Y230F	82.2 ± 6.1
D232H	31.1 ± 3.2
D232N	36.1 ± 3.3
D232E	76.2 ± 2.3
D232A	NFB‡
Combined αIIb substitutions (rat→human)
V156D,D232H	39.1 ± 11.4
W3:4-1, D232H	30.9

^*Recombinant αIIbβ3 was expressed in CHO cells and was activated using DTT as described in “Fibrinogen binding to CHO cells expressing αIIbβ3.”

^†The results are the mean and 1 SE of three or more experiments.

^‡NFB indicates no fibrinogen binding.