Expression of ADAMTS13 and its truncation variants in HEK 293T cells. (A) The predicted domain structure of ADAMTS13 (WT) and the range of amino acid sequences encompassed in the 5 truncated constructs (AD2, AD5, AD8, and AD13) used in this study. (B) Expressed ADAMTS13 and its truncation variants in either cell lysate (Cell) and/or concentrated culture medium (Medium) were digested with PNGase F (F) or endoglycosidase H (H) and analyzed by SDS-PAGE and immunoblotting with anti-V5. C indicates control proteins not digested with either enzyme. The numbers to the left of the gels indicate the position of molecular size markers in kilodaltons. The cell lysate data are not shown for AD2, AD8, and AD13. The space in AD5 and AD13 indicates repositioned gel lanes.