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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Cell Signal. 2008 Nov 5;21(2):301–308. doi: 10.1016/j.cellsig.2008.10.017

Figure 1. Endogenous and overexpressed AC6 are co-localized in lipid rafts independent of caveolin-1 expression.

Figure 1

Untreated COS-7 or HEK-293 cells (control) or cells incubated with an adenovirus expressing human AC6 for 24 h (Adv-AC6) were fractionated using a non-detergent method to isolate lipid rafts and caveolae (see Methods). Following sucrose density centrifugation, 10 fractions were collected and fractions 2–10 were separated by SDS-PAGE and analyzed by immunoblot using either anti-caveolin-1 or anti-his tag and S-protein. Images shown are representative of 4 experiments.