Table 2.
Human PHOX2B elements conserved to zebrafish phox2b locus demonstrate activity consistent with orthologous zebrafish sequences.
| Human Amplicon | Expression (HCS) |
Zebrafish Amplicon | Expression (ZCS) |
Coincident Control |
| PHOX2B-HCS+0.6* | Fb, CG | phox2b-ZCS +0.5 | Hb, SC | No |
| PHOX2B-HCS -0.0 | VDi, Hb | phox2b-ZCS +0.0 | Hb, SC | Yes |
| PHOX2B-HCS -2.9* | Fb, Hb | phox2b-ZCS -1.4 | Mb, Hb, SC | Yes |
| PHOX2B-HCS -73.5 | OT, CG, Hb | phox2b-ZCS -8.3 | Mb, Hb, CG | Yes |
| PHOX2B-HCS -108.3 | Fb, Hb | phox2b-ZCS -16.6 | Mb, Hb | Yes |
| PHOX2B-HCS-114.8 | Fb, Hb, SC | phox2b-ZCS-20.1 | Hb | Yes |
| PHOX2B-HCS -116.7 | CG | phox2b-ZCS -23.7 | Hb, SC, ENS | No |
| PHOX2B-HCS -130.4 | ENS | phox2b-ZCS -23.7 | Hb, SC, ENS | Yes |
HCS expression is pattern driven by human amplicon in G0 zebrafish embryos (* indicates G1 expression pattern). ZCS expression is pattern driven by orthologous zebrafish amplicon in G1 zebrafish embryos [29]. Overlap is categorized as Yes, tissue overlap in expression patterns, but additional tissues seen; No, no overlap in expression. Fb, Forebrain; OT, Oculomotor and trochlear motor Progenitors; VDi, Ventral diencephalon; Hb, Hindbrain; CG, Cranial ganglia,; SC, Spinal cord ENS, Enteric nervous system.