Table 2.

Mean association and dissociation kinetic rate constants (k_on, k_off) and equilibrium dissociation constants (KD), calculated from surface plasmon resonance (SPR) measurements for aqueous Congo red (CR) flowing past chip-linked FP and Aβ(26–42) monolayers^a

Chip-linked peptide	kon (1/Ms)	koff (1/s)	KD (nM)
FP	62.9 × 105	18.7 × 10−3	2.97
Aβ(26–42)	19.2 × 105	4.0 × 10−3	2.10

^aCongo red in HBS-EP buffer was flowed past monolayers of FP or Aβ(26–42), linked via their respective N-terminal amides to CM5 sensor chips in a Biacore 3000 system (see section 2). Mean kinetic rate constants (k_on, k_off) and equilibrium dissociation constants (KD = k_off/k_on) were determined from curve fitting analyses of SPR results at 10 nM CR.