Figure 1. H3K27 methylation in low pass Eed^null ES cells.

(A.)Wild-type, low pass Eed^null, and high pass Eed^null ES cells were stained with an antibody against H3K27me1. Monomethylation is robust in wild-type ES cells and detectable in low pass Eed^null ES cells, but absent in high pass mutant ES cells. (B.) Low pass Eed^null ES cells were stained with antibodies against H3K27me1, me2, and me3. Low pass mutant ES cells stain positively for H3K27me1, but not for H3K27me2 or me3. Positively staining feeder cells serve as internal controls. Corresponding gray-scale images of DAPI stains are shown to the right.